Review





Similar Products

dh5α  (New England Biolabs)
99
New England Biolabs dh5α
Dh5α, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/pmc12796543-239-6-7?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
dh5α - by Bioz Stars, 2026-07
99/100 stars
  Buy from Supplier
endura competent e coli cells  (Lucigen Corp)
86
Lucigen Corp endura competent e coli cells
Endura Competent E Coli Cells, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/us12655403-1902-7-12?v=Lucigen+Corp
Average 86 stars, based on 1 article reviews
endura competent e coli cells - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
competent e coli overexpress c41 de3 cells  (Lucigen Corp)
86
Lucigen Corp competent e coli overexpress c41 de3 cells
Competent E Coli Overexpress C41 De3 Cells, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/us12655176-425-8-14?v=Lucigen+Corp
Average 86 stars, based on 1 article reviews
competent e coli overexpress c41 de3 cells - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
competent c41 de3 e coli cells  (Fisher Scientific)
86
Fisher Scientific competent c41 de3 e coli cells
Competent C41 De3 E Coli Cells, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/pm42301917-58-1-6?v=Fisher+Scientific
Average 86 stars, based on 1 article reviews
competent c41 de3 e coli cells - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
ecos competent e coli bl21 de3  (Nippon Gene Co Ltd)
86
Nippon Gene Co Ltd ecos competent e coli bl21 de3
Ecos Competent E Coli Bl21 De3, supplied by Nippon Gene Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/us12655144-405-16-22?v=Nippon+Gene+Co+Ltd
Average 86 stars, based on 1 article reviews
ecos competent e coli bl21 de3 - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
e coli bl21 de3 competent cells  (Sangon Biotech)
86
Sangon Biotech e coli bl21 de3 competent cells
E Coli Bl21 De3 Competent Cells, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/pm42257678-212-0-25?v=Sangon+Biotech
Average 86 stars, based on 1 article reviews
e coli bl21 de3 competent cells - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
bl21 de3 e coli competent cells  (Sangon Biotech)
86
Sangon Biotech bl21 de3 e coli competent cells
Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).
Bl21 De3 E Coli Competent Cells, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/pmc13280793-36-8-16?v=Sangon+Biotech
Average 86 stars, based on 1 article reviews
bl21 de3 e coli competent cells - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
competent e coli bl21  (Fisher Scientific)
86
Fisher Scientific competent e coli bl21
Expression and purification of Nb16-β-gal. <t>BL21</t> bacteria carrying pRCH-Nb16-βGal were used to produce the target protein. Proteins in lysates from uninduced (lane U) and induced (lane I) bacteria, fractions containing the target protein after Ni 2+ column purification (lane 1), and the purified target protein after the second-step SEC purification (lane 2) were analyzed by SDS-PAGE on a 4–12% SDS gel. The molecular masses (in kDa) of the proteins in the protein standards (lane M) are indicated on the right side of the gel image. The horizontal arrow indicates the Nb16-β-gal band.
Competent E Coli Bl21, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/pmc12860646-78-0-7?v=Fisher+Scientific
Average 86 stars, based on 1 article reviews
competent e coli bl21 - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier
e coli dh5α competent cells  (Sangon Biotech)
86
Sangon Biotech e coli dh5α competent cells
Expression and purification of Nb16-β-gal. <t>BL21</t> bacteria carrying pRCH-Nb16-βGal were used to produce the target protein. Proteins in lysates from uninduced (lane U) and induced (lane I) bacteria, fractions containing the target protein after Ni 2+ column purification (lane 1), and the purified target protein after the second-step SEC purification (lane 2) were analyzed by SDS-PAGE on a 4–12% SDS gel. The molecular masses (in kDa) of the proteins in the protein standards (lane M) are indicated on the right side of the gel image. The horizontal arrow indicates the Nb16-β-gal band.
E Coli Dh5α Competent Cells, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/competent+e+coli/pmc13261013-51-8-32?v=Sangon+Biotech
Average 86 stars, based on 1 article reviews
e coli dh5α competent cells - by Bioz Stars, 2026-07
86/100 stars
  Buy from Supplier

Image Search Results


Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

Journal: Frontiers in Veterinary Science

Article Title: Construction and immunogenicity evaluation of a bivalent nanoparticle based on mi3 displaying porcine circovirus type 2 and type 3 capsid proteins

doi: 10.3389/fvets.2026.1862938

Figure Lengend Snippet: Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

Article Snippet: The pET28a(+) plasmid for recombinant protein expression and BL21(DE3) E. coli competent cells were purchased from Sangon Biotech (Shanghai) Co., Ltd.

Techniques: Protein Purification, Clone Assay, Expressing, Purification, Recombinant, Sonication, Western Blot, Biomarker Discovery

Expression and purification of Nb16-β-gal. BL21 bacteria carrying pRCH-Nb16-βGal were used to produce the target protein. Proteins in lysates from uninduced (lane U) and induced (lane I) bacteria, fractions containing the target protein after Ni 2+ column purification (lane 1), and the purified target protein after the second-step SEC purification (lane 2) were analyzed by SDS-PAGE on a 4–12% SDS gel. The molecular masses (in kDa) of the proteins in the protein standards (lane M) are indicated on the right side of the gel image. The horizontal arrow indicates the Nb16-β-gal band.

Journal: Food Chemistry: Molecular Sciences

Article Title: Usage of nanobody-beta-galactosidase fusion in immunoassays and its application in detecting a peanut allergen

doi: 10.1016/j.fochms.2026.100357

Figure Lengend Snippet: Expression and purification of Nb16-β-gal. BL21 bacteria carrying pRCH-Nb16-βGal were used to produce the target protein. Proteins in lysates from uninduced (lane U) and induced (lane I) bacteria, fractions containing the target protein after Ni 2+ column purification (lane 1), and the purified target protein after the second-step SEC purification (lane 2) were analyzed by SDS-PAGE on a 4–12% SDS gel. The molecular masses (in kDa) of the proteins in the protein standards (lane M) are indicated on the right side of the gel image. The horizontal arrow indicates the Nb16-β-gal band.

Article Snippet: Competent E. coli BL21 (BL21 StarTM (DE3), Fisher Scientific) was transformed with each of the plasmids described above.

Techniques: Expressing, Purification, Bacteria, SDS Page, SDS-Gel